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KMID : 0545120040140020367
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 2 p.367 ~ p.372
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Dithiothreitol Attenuates the Pathogenic Interaction Between pseudomonas aeruginosa and Drosophila melanogaster
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LEE, JI-SUN
KIM, SEOL-HEE/CHO, YOU-HEE
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Abstract
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Infection of Drosophila melanogaster adults with Pseudomonas aeruginosa (PA14) can kill the flies within 48 h. We found that the virulence of PA14 was significantly attenuated when treated with a reducing agent, dithiothreitol (DTT), prior to infection. Infection with DTT-treated PA14 elevated Metchnikowin expression at 22 h post-infection and the virulence of DTT-treated PA14 was not attenuated in Dif and Relish mutants. These results suggest that DTT pre-treatment of PA 14 can aggravate certain virulence factors that may be required to paralyze fly immune responses, triggering Metchnikowin expression via Dif and Relish activations.
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